Xenbase Image ID: 81343

Fig. 5. Retinoic acid (RA) signaling directly alters ski expression in vivo. A: Whole-mount in situ hybridization of embryos pretreated with 20 min of 1 mM cycloheximide to block translation followed by 1 hr treatment with 1 mM RA. In a dorsal view, embryos treated from St. 146 showed increased staining with RA even in the presence of cycloheximide. Embryos treated from St. 268 (side view) showed the same result. B: Quanti- tative reverse transcriptase-polymerase chain reaction (qRT-PCR) of similarly treated embryos between stages 14 and 16 showed that ski expres- sion is up-regulated by RA even in the presence of cycloheximide. C: qRT-PCR analysis of cyp26a1, a known direct target of RA signaling, was used as a positive control for the RA and cycloheximide treatments. Error bars represent standard error of the mean. n1⁄45. *P < 0.05, **P < 0.01, ***P < 0.001. Image published in: Melling MA et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page