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Fig. 7. Overexpression of both Ski and the Ski-APRG mutant causes decreased expression of CYP26A1. A: Western blot (anti-FLAG) confirming protein synthesis from the Ski and Ski-APRG constructs after transfection into HaCaT cells. B: Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) quantifying the levels CYP26A1 mRNA shows a decreased level of expression after transfection with either Ski construct. C: qRT-PCR of similarly transfected cells quantifying the levels of ID3 mRNA, a target of bone morphogenetic protein (BMP) signaling, showing that there is decreased levels of expression with Ski over expression but not with over expression of the Ski-APRG construct. Error bars represent standard error of the mean. mRNA levels were normalized to the GAPDH mRNA levels. n 1⁄4 3. *P < 0.05, **P < 0.01. Image published in: Melling MA et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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