Xenbase Image ID: 81355

Fig. 7. Inhibition of ductin causes changes in voltage patterns that correlate with CF phenotypes. A: Stills of wildtype embryos showing the pattern of Vmem in the anterior and antero-dorsal regions of neurulae at approximately stage 18. Arrowheads point to the most consistent features of the pattern (11 of 11 uninjected embryos). A.i: Green arrows point to depolarized neural folds. Yellow arrows point to hyperpolarization in the future olfactory/oral region. A.ii: Green arrow points to the disappearing signal from the neural tube floor. A.iii: Blue asterisks mark hyperpolarized ectodermal regions lateral to the neural folds. B: The pattern of pH in the anterior and antero-dorsal regions of untreated neurulae. Arrows point to features of a normal pattern, and are the same as Vmem shown in A. C, D: Abnormal regionalization of Vmem and pH caused by injection of xductnoTM4. C.i: Abnormal Vmem patterns were seen in 9 of 13 injected embryos. Green arrowheads point to the normal depolarized neural folds, red arrows to the abnormal hyperpolarized side. D.i: Red lines indicate the abnormal angle between the high pH lateral ectoderm and the low pH anterior folds. C.ii, D.ii: The same embryos imaged at stage 45 display gross malformations (red arrowheads) in the areas with abnormal Vmem or pH. Image published in: Vandenberg LN et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xla Wt + atp6v0cdel + NF18 (whole-mount microscopy) Fig 7 C i Anatomical Phenotype abnormal establishment of tissue polarity Xla Wt + atp6v0cdel + NF45 (whole-mount microscopy) Fig 7 C ii Anatomical Phenotype abnormal anatomical region morphology abnormal Meckel's cartilage morphology abnormal optic vesicle morphology Xla Wt + atp6v0cdel + NF18 (whole-mount microscopy) Fig 7 D i Anatomical Phenotype abnormal pre-chordal neural plate morphology decreased width of the pre-chordal neural plate Xla Wt + atp6v0cdel + NF45 (whole-mount microscopy) Fig 7 D ii Anatomical Phenotype abnormal eye morphology abnormal pre-chordal neural plate morphology abnormally localised eye increased pigmentation in the optic stalk Larger Image Printer Friendly View

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