Xenbase Image ID: 81358

Supporting Figure 2 Lineage tracers distinguish characteristics of ductin inhibition on placodal versus neural crest derived organs. Embryos were co-injected with a β-gal lineage tracer plus xduct-noTM4, or they were injected with xduct-YFP. In both cases, one blastomere of 2-cell embryos was injected. A: Embryo co-injected with xduct-noTM4 and β-gal has an eye attached directly to the brain. The affected eye (blue arrow) is on the same side as the injection, as was observed in almost all cases. B: An embryo co-injected with xduct-noTM4 and β-gal with small, underdeveloped jaws and BA on both sides (red arrows). The β-gal signal indicates that only one side of this embryo actually received injected mRNA. C: To account for possible differences in the localization of β-gal lineage tracer and ductin mutant mRNA, we further verified these results using a fluorescently labeled xduct construct (xduct-YFP). C.i.: Brightfield image. Cii: Fluorescent signal from YFP. Again, eye phenotypes (blue arrow) were observed only on the injected side. This embryo also has abnormal growth of brain (white arrows), which is clearly labeled with YFP. Image published in: Vandenberg LN et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xla Wt + atp6v0cdel + NF45 (whole-mount microscopy) Fig S2 A Anatomical Phenotype fused eyes Xla Wt + atp6v0cdel + NF45 (whole-mount microscopy) Fig S2 B Anatomical Phenotype decreased size of the branchial arch skeleton decreased size of the Meckel's cartilage Xla Wt + atp6v0cdel + NF45 (whole-mount microscopy) Fig S2 C i ii Anatomical Phenotype abnormal brain morphology abnormal optic vesicle morphology Larger Image Printer Friendly View

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