Xenbase Image ID: 81360

Supplemental Fig. 1. Embryos injected with mRNA encoding β-gal and untagged δLef1 (i, iii, v) or untagged δXTcf3 (ii, iv, vi) were examined by in situ hybridization for expression of Sox3 (i, ii); Slug (iii, iv); and C-myc (v, vi). All embryos are positioned showing anterior/dorsal side, with their injected side to the right (arrow). Red staining is lineage tracer β-gal. δXTcf3 potently expands the neural plate marker, Sox3, and strongly inhibits markers of the neural crest (Slug and C-myc) and placodal regions (C-myc). At equivalent levels of injected mRNA, δLef1 has little or no effect on expression of these markers. These findings are fully consistent with results using epitope tagged forms of these proteins. Image published in: Heeg-Truesdell E and LaBonne C (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 14 neuroectoderm neural plate snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural crest cranial neural crest myc.S c-myc, cmyc, myc-a, myc-b, X-cmyc, Xcmyc X. laevis Throughout NF stage 18 anterior neural fold preplacodal ectoderm anterior placodal area Image source: Published Larger Image Printer Friendly View

Return to previous page