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Fig. 4. Maternal Dnd1 is required for vegetal cortical microtubule assembly. (A,B) Confocal images showing the formation of vegetal cortical microtubules in fertilized eggs (A, 55 minutes post-fertilization; top and bottom figures) and in artificially activated eggs (B, 40 minutes post-needle pricking). Fixed eggs were bisected and the vegetal halves were stained with an anti-Tubulin antibody. Formation of vegetal cortical microtubules was severely disrupted in all Dnd1 knockdown fertilized eggs. In 27% of Dnd1 knockdown fertilized eggs (A, lower middle), a few microtubules were detected (indicated by arrows). Also note that at 40 minutes post-needle pricking, disordered microtubules just begin to polymerize into the vegetal cortex of activated eggs (supplementary material Fig. S4). Image published in: Mei W et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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