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Fig. S5. Effects of Dnd1 overexpression. (A) Confocal images showing the formation of vegetal cortical microtubules in control and dnd1 (500 pg) overexpressed host-transfer embryos (45 minutes post-fertilization). (B) Effect of Dnd1 overexpression on wnt11 polyadenylation. Total RNA from control and Dnd1 overexpressed embryos (eight-cell stage) was reverse transcribed with oligo dT (left) and random hexamers (dN6, right). Oligo dT- and random-primed cDNAs were used as templates for PCR with wnt11 and Emi1 primers. (C) RT-PCR showing that overexpression of dnd1 (500 pg) had no detectable effect on the expression of dorsal genes. dnd1 RNA was injected into oocytes, allowed to translate and these oocytes were used for host transfer. Resulting embryos were harvested at stage 10.5 and analyzed for expression of noggin, chordin, Xnr3,siamois and sizzled. (D) Morphology of control (blue) and Dnd1-overexpressed host-transfer embryos (red). Image published in: Mei W et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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