Xenbase Image ID: 81394

Fig. 2. The Morpholino antisense oligonucleotide specifically inhibits the translation of the xEmGCNF mRNA. Embryos were injected at the two-cell stage with 42 ng of a standard morpholino oligonucleotide as control (STA: lane 2) or a xEmGCNF-specific antisense Morpholino oligonucleotide (MOR: lanes 3) into each blastomere. Protein extracts of injected embryos at stage 17 were analyzed by Western blotting, using an antiserum against xEmGCNF [DEF] (David et al., 1998). The positions endogenous xEmGCNF (arrow) is indicated at the left. The lowest band (arrowhead) detected by the antiserum is non-related to GCNF and can be used as a loading control. Image published in: Barreto G et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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