XB-IMG-81397
Xenbase Image ID: 81397
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Fig. 5. Function of xEmGCNF is required for normal hindbrain development. (A) Whole mount in situ hybridization of neurula embryos at stages 17 and 20 to
detect the expression of the anterior marker Otx2, the posterior marker Gbx2, the marker for rhombomeres 3 and 5 Krox20 and the MHB marker En2. Albino
embryos (1 MOR, right panels) were unilaterally injected at the two-cell stage with 42 ng of the MOR and 150 pg b-galactosidase mRNA as lineage tracer.
The injected side is to the right as identified by the red b-gal staining. The inset shows Krox20 expression pattern after unilateral depletion of xEmGCNF
without b-gal staining. Non-injected control embryos (CON, left panels) show the normal expression patterns of each marker. (B, C) Depletion of xEmGCNF
causes a hindbrain-specific inhibition of RARg transcription. (B) Dorsal, posterior, and anterior aspects of a stage 18 neurula embryo injected with 42 ng of
MOR into one blastomere at the two-cell stage. The injected side (to the right) was identified by lineage tracing. (C) Lateral and dorsal aspects (lowest panels)
of whole mount in situ to detect RARg expression in stage 35 embryos that were injected at the two-cell stage into each blastomere with 42 ng of STA (left
panels) or MOR (right panels). Image published in: Barreto G et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
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