Xenbase Image ID: 81438

Fig. 3. Dmrt5 is upregulated by attenuation of BMPs and downregulated by overexpression of the proneural factors Ngnr1, Ebf2, Ebf3 and Ath5. (A) Real time RT-PCR analysis of animal cap explants isolated from embryos injected with increasing doses of Noggin mRNA (40 pg, 100 pg and 400 pg) and collected at stage 17. Note that Dmrt5 expression is activated by higher doses of Noggin mRNA as compared to Dmrt4. Each value has been normalized to the level of GAPDH expression. (B) Overexpression of MT-Ngnr1, MT-hGR-Ebf2, MT-hGR-Ebf3 and MT-Ath5 (400 pg) blocks Dmrt5 and Dmrt4 in the embryo. Anterior views of whole-mount in situ stained embryos are shown, with dorsal to the top. In all cases, LacZ mRNA was used as a lineage tracer to identify the injected side. Image published in: Parlier D et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue dmrta1.S dmrt4, XDmrt4 X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate dmrta2.S dmrt5 X. laevis Throughout NF stage 17 neural plate pre-chordal neural plate border Image source: Published Larger Image Printer Friendly View

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