Xenbase Image ID: 81452

Figure 4. xSuv4-20h1/h2 enzymatic activity is required in the ectodermal germ layer. (A, D) Schematic illustrations of targeting microinjections into mesendodermal or ectodermal territories at 8-cell stage. (B) Injecting xSuv4-20h MOs into the mesendoderm causes no apparent morphological phenotype in the embryo. (C) Neural, mesodermal and endodermal marker genes are expressed normally. (E) xSuv4-20h MOs reduce eyes, cranial and trunk melanophores, when injected into the ectoderm. (F) Expression of all tested markers in mesoderm and endoderm is normal, except for Delta-like 1, whose expression specifically in the open neural plate is strongly reduced on the injected side. Global morphology was assessed at hatching stage (NF36), molecular markers at indicated stages during neurulation. Top row images in (B) and (E) depict whole embryos for overview. doi:10.1371/journal.pgen.1003188.g004 Image published in: Nicetto D et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 13 neuroectoderm neural plate circumblastoporal collar neural progenitor cell neural plate border myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 mesoderm presomitic mesoderm circumblastoporal collar sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 15 endoderm Image source: Published Larger Image Printer Friendly View

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