Xenbase Image ID: 81458

Figure S5. Germ layer marker gene expression in xSuv4-20h double morphants. (A) Immuno-histochemistry on ctrl-MO and xSuv4-20h double-morphant tadpoles. Panels show representative cross-sections of neural tubes stained with antibodies against the histone epitopes indicated on top. Inj injected side. Squares on double-morphant sections represent the croped pictures shown in Figure 1D. (B-F) RNA in situ hybridization analysis of ctrl-MO injected and double morphant embryos at the indicated stages using probes against Chordin, Xnr-3 and Gooscoid (B), Krox20 and Otx2 (C), Pax-6 (D), N-CAM (E), FoxD5, Geminin, Zic2, Zic3, Sox3, Sox11 and VegT (F). Chordin, Xnr-3 and Gooscoid dorsal side views; animal pole is on the top. Krox20 - dorsal views, anterior on the left. Otx2 - anterior views, dorsal on the top. Pax-6 head region; rescued embryos included. N-CAM - dorsal views of stained embryos with the anterior on the left; rescued embryos included. FoxD5, Geminin, Zic2, Zic3 - dorsal views; injected halves are lineage-traced by coinjection of LacZ mRNA and subsequent βal staining (light blue). Sox2, Sox3 - dorsal views, anterior to the left. VegT - internal stain in bisected embryos. Image published in: Nicetto D et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 dorsal marginal zone nodal3.1.L nr3, Xnr-3, Xnr3 X. laevis Throughout NF stage 10.5 dorsal marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.5 dorsal marginal zone egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 20 presumptive rhombomere otx2.L otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 20 optic vesicle neuroectoderm stomodeal-hypophyseal primordium neural plate pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Sometime during NF stage 29 and 30 to NF stage 33 and 34 retina midbrain forebrain hindbrain ncam1.L n-cam, n-cam-1, ncam, ncam1-a, ncam1-b X. laevis Sometime during NF stage 19 to NF stage 20 optic vesicle neural fold presumptive rhombomere foxd4l1.1.L foxd4, Foxd4l1, foxd4l1.1-a, foxd4l1.1-b, foxd5, foxD5a, FoxD5b, Xfd-12, XFlip, xlFoxD5a, xlFoxD5b, xtFoxD5 X. laevis Throughout NF stage 11 dorsal marginal zone gmnn.L gem, geminin X. laevis Throughout NF stage 11 marginal zone zic2.L hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 X. laevis Throughout NF stage 11 marginal zone zic3.L xzic3, Zinc finger protein of the cerebellum 3 X. laevis Throughout NF stage 11 marginal zone sox3.L Sry, Xlsox3, xSox3, Xtsox3 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate sensorial layer sox11.L sox11-a, sox11-b, XLS13, xls13a, XLS13b, xSOX-11, xsox11 X. laevis Sometime during NF stage 15 to NF stage 16 sensorial layer neural plate neuroectoderm vegt.L antipodean, brat, tVegT, vegt-a, vegt-b, xombi X. laevis Throughout NF stage 11.5 mesoderm marginal zone involuting marginal zone Image source: Published Larger Image Printer Friendly View

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