Xenbase Image ID: 81460

Figure S7. A functional SET domain is required for morphological and molecular rescue of double-morphants phenotypes. (A) Morphological phenotypes of NF30-33 double morphants (A), embryos injected with xSuv4-20h1, h2 morpholinos and active (active rescue, B), or inactive (inactive rescue, C) mouse Suv4-20h1, h2 mRNAs. Embryos were coinjected in one half at two cell stage with Alexa Fluor 488 Dextran as lineage tracer (green channel) to identify the injected side and sort embryos. (D) Penetrance of the eye phenotype in the indicated samples. Displayed are the results from two independent experiments. (E) RNA In situ hybridization analysis of NF15 uninjected, double morphants, active and inactive rescue embryos using probes against Oct-25, N-tubulin and MyoD. The pictures show dorsal view of stained embryos, anterior is on the left. Image published in: Nicetto D et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 15 neural plate notoplate presumptive midbrain-hindbrain boundary tubb2b.L N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 15 Rohon-Beard neuron neural plate neural progenitor cell myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 presomitic mesoderm paraxial mesoderm Image source: Published Larger Image Printer Friendly View

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