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Figure S9. Mus musculus Suv4-20h1 or h2 mRNA overexpression. (A) Western Blot analysis of uninjected embryos or embryos injected with Mus musculus Suv4-20h1 or h2 mRNAs at different concentrations. Bulk histones from NF11.5 embryos were isolated and analyzed as described in Materials and Methods section. Pan H3 antibody was used as loading control. (B, C) Morphological phenotypes of NF30-33 embryos injected with mouse Suv4-20h1 (B) or h2 (C) mRNA. (D) RNA In situ hybridization analysis of uninjected embryos (top row) and embryos injected with mSuv4-20h1 (middle row) or h2 (bottom row) mRNA using probes against Ngnr 1a, Delta-like 1 and Rx-1. Rx-1 pictures show the head of NF30-33 stained embryos. Ngnr 1a (NF12.5) and Delta-like 1 (NF13) pictures show dorsal view of stained embryos, anterior is on the left. Image published in: Nicetto D et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue neurog2.L atoh4, neurog2-a, neurog2-b, neurogenin 2, neurogenin-2, neurogenin-related-1, neurogenin2, ngn-2, ngn2, ngnr, ngnr 1a, Ngnr-1, Ngnr1, X-ngnr-1, xngn2, Xngnr-1, XNgnr1 X. laevis Throughout NF stage 12.5 sensorial layer neural plate neural plate border neuroectoderm dll1.L delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 X. laevis Throughout NF stage 13 circumblastoporal collar neural progenitor cell neural plate neural plate border rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Sometime during NF stage 29 and 30 to NF stage 33 and 34 retina pineal gland Image source: Published Larger Image Printer Friendly View

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