Xenbase Image ID: 81513

Fig. 6. VPA-mediated developmental defects are due to inhibition of class I HDACs. A: runx1 expression in the anterior ventral region of late neu- rula stage control embryos (stage 18). B, C: TSA (B) and VPA (C) suppress runx1 expression. D: The inactive VPA analog VPM does not inhibit runx1 expression. E: bmp4 mRNA (2 ng) induction of globin expression in ectodermal explants is blocked by TSA (bmpSA). F: VPA (class I HDAC inhibitor), CI-994 (selective inhibitor of HDACs 1, 2, and 3), and C60 (IC50 for HDACs 1 and 2>>HDAC3) increase global acetylation of his- to>>H3 at lysine 9/14 (H3K9/14Ac) at 24 hr post-treatment, as demonstrated by Western blotting. Bottom: Total H3 (loading control). G: Lateral view of stage-45 tadpoles treated with vehicle (DMSO) control (G), C60 (H), VPA (I), and CI-994 (J) from late neurula (stage 19) to tailbud (stage 31) stages. Image published in: Shah RR et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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