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Fig. 7. Runx1 expression rescues VPA-mediated hematopoietic defects. A: Benzidine staining of hemoglobin (blue) in untreated stage-43 tad- pole. B: Treatment with VPA inhibits RBC development. C: Injection of runx1 mRNA partially recovered erythrocyte development in VPA-treated embryos. D: Percentage of benzidine-positive tadpoles for uninjected (n1⁄420), VPA-treated (n1⁄425), VPA-treated runx1 (n1⁄421), and runx1 expres- sion alone (n1⁄421). This experiment was repeated with 4 separate clutches of embryos and the table shows data from all 4 experiments. Intensity of benzidine stain was reduced in VPA and VPAunx1 groups (as shown in C). E: Expression of globin was detected by WISH in all (23/23) control tadpoles, 0/20 VPA-treated tadpoles, and 7/18 VPA-treated, runx1-expressing tadpoles. Image published in: Shah RR et al. (2013) Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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