Xenbase Image ID: 81559

Figure 1. Generation of transgenic LG clones. (a) General schema of the production of transgenic LG clones. LG female clones produce both diploid (large) and aneuploid (small) eggs. The small eggs were discarded while the diploid eggs were activated using UV‐irradiated sperm. After activation, eggs were microinjected with the different constructs for transgenesis. Embryos were allowed to develop and were screened for GFP expression at stage 56 using a fluorescence stereomicroscope. Transgenic tadpoles were raised to adulthood to obtain F1 progeny. (b) Representative images of live nontransgenic (a) and transgenic larvae. Dejellied fertilized LG eggs were co‐injected with (b) 1 ng of phiC31 integrase mRNA and 25 pg of CMV‐GFP‐DI‐attB vector, (c) 0.1 ng of SB mRNA and 15 pg of HSV‐GFP, (d) 1×10–3 I‐SceI units and 80 pg of I‐SceI–GFP in a total volume of 10 nl per egg. Reproduced with permission of the Publisher, John Wiley & Sons. Image published in: Nedelkovska H and Robert J (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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