XB-IMG-81596
Xenbase Image ID: 81596
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Figure 6. ephrinB1 Is Necessary for Retinal Progenitors to Move into the Eye Field(A) Embryos were injected with ephrinB1 mRNA alone, or plus an ephrinB1 morpholino (MO), inverted ephrinB1-MO (invert ctl), or standard control MO (std ctl), and analyzed by Western blot. The ephrinB1-MO does not block the translation of a 5′ truncated ephrinB1 (ephrinB1δUTR) or of ephrinB3, which is recognized by the same antibody.(B and C) ephrinB1-MO injected into D1.1.1 (green) represses population of retina.(D) Percentage of embryos with D1.1.1 clones in retina. ephrinB1-MO caused a dose-dependent reduction (p < 0.05 at all doses).(E) ephrinB1-MO injection into D1.1.1 represses pax6 expression (arrow).(F) Ectopic expression of pax6 (red) does not induce ectopic ephrinB1 (blue).(G) Expression of pax6 in an ephrinB1-MO-injected embryo restores D1.1.1 clone (green) to retina.(H) Ectopic expression of ephrinB1 (red) does not induce ectopic pax6 expression (blue).
Image published in: Moore KB et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |