XB-IMG-81598
Xenbase Image ID: 81598
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Figure S2. (A) Animal cap explants injected with noggin mRNA expressed rx1, pax6, and ephrinB1. Coexpression of cFGFR2 did not repress this expression, nor did added expression of ephrinB1 alter it. WE, whole embryo sample at stages 14 or 15/16. EF1a served as a loading control. T, without reverse transcription step. ctrl, uninjected animal cap explants.(B) Animal cap explants injected with noggin mRNA expressed rx1 and ephrinB1, and coexpression of cFGFR2 did not repress this expression. Ctrl, uninjected animal cap explants.(C) Injection of ephrinB1-MO into blastomere D1.1.1 repressed rx1 expression (blue) on side of injection (arrow).(D) Ectopic expression of rx1 (red) either in neural plate (left; frontal view) or in lateral epidermis (right; side view) did not induce ectopic ephrinB1 expression (blue).(E) Ectopic expression of ephrinB1 (red) either in neural plate (left; frontal view) or in lateral epidermis (right; side view) did not induce ectopic or alter the endogenous rx1 expression domain (blue).
Image published in: Moore KB et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |