Xenbase Image ID: 81664

Fig. 1. Fibronectin fibrils surround the somitic mesoderm during gastrulation and extension. A,B: Sagittal sections through the future anterior midline of the early- (A, stage 10 ) and mid-gastrula (B, stage 11 ) show fibronectin fibrils along the neural/mesoderm interface into the cleft of Brachet (arrowheads) but not at the mesoderm/ endoderm interface (arrows). C: A projection of 251 transverse confocal sections through the dorsal mesoderm at stage 13 (C) shows that fibronectin appears at the somitic mesoderm/endoderm interface (arrow) in addition to the neural/somitic mesoderm boundary (arrowhead). At the same time, fibrils are cleared from the ventral (v) and are in the process of being cleared from the dorsal (d) surfaces of the notochord (no). D: Progressive clearing of fibronectin from the notochord is better seen in projections of en face confocal stacks of an early immature state (D), to a more connected network (E), and finally to a more tightly connected array surrounding the somitic mesoderm (F, so) when fibrils are cleared from both surfaces of the notochord (no). G: Projections of subsets of slices from a single en face stack containing only the neural/mesoderm boundary (G) or the mesoderm/endoderm boundary (H) show distinct fibrils, whereas a projection of confocal sections through the middle of the mesoderm (shown overexposed in I) show fibrils at the lateral walls of the notochord (no) and small fibronectin-rich puncta within the somitic mesoderm (so). J: Ontogeny of the fibrillar fibronectin matrix. A ut-awaytransverse view of fibrillar fibronectin matrix assembly from mid- to late-gastrula stages. bp, blastopore; ne, neural ectoderm; en, endoderm; a, anterior; p, posterior. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.] Image published in: Davidson LA et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue fn1.S fibronectin, gene C, LOC121393057, MGC97508 X. laevis Throughout NF stage 10.25 to NF stage 12 involuted dorsal mesoderm presomitic mesoderm fn1 fibronectin, gene C, LOC121393057, MGC97508 X. tropicalis Throughout NF stage 13 mesoderm notochord neural plate somite Image source: Published Larger Image Printer Friendly View

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