Xenbase Image ID: 81670

Figure 4. Ectopic and early induction of beta cells by Ngn3-GR. Insulin expression in tadpoles that have been sectioned through the dorsal pancreas, with the head removed. (a) Control injected embryos. Endogenous insulin expression is first detected at Stage 32 in a small dorsal domain. Endogenous insulin expression in dorsal pancreas (dp) is labeled by arrow in panel (c). (e) Ngn3-GR injected embryos treated with Dex for 4 h at Stage 12 showed increased insulin expression at Stage 28 (n = 14/18), Stage 30 (n = 24/30), and Stage 32 (n = 46/56). (h) Side view of Stage 34 tadpole treated with Dex showing the extent of ectopic insulin expression (line). Image published in: Oropeza D and Horb M (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue ins.S ins-a, ins-b, ins1, insulin, insulin I, preproinsulin, xins X. laevis Throughout NF stage 32 pancreas dorsal pancreatic bud Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + neurog3-GR + DEX + NF28 (in situ hybridization) Fig 4 e Expression Phenotype mislocalised ins.L expression in foregut endoderm increased amount ins.L expression in foregut endoderm Xla Wt + neurog3-GR + DEX + NF32 (in situ hybridization) Fig 4 f Expression Phenotype mislocalised ins.L expression in dorsal pancreatic bud increased amount ins.L expression in dorsal pancreatic bud Xla Wt + neurog3-GR + DEX + NF32 (in situ hybridization) Fig 4 g Expression Phenotype mislocalised ins.L expression in dorsal pancreatic bud increased amount ins.L expression in dorsal pancreatic bud Xla Wt + neurog3-GR + DEX + NF33/34 (in situ hybridization) Fig 4 h Expression Phenotype mislocalised ins.L expression in foregut endoderm increased amount ins.L expression in foregut endoderm Larger Image Printer Friendly View

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