Xenbase Image ID: 81698

FIG. 1. Tol2 transgenesis in Xenopus tropicalis. (a) Schematic of the Tol2 transposon (Tol2) and the modified non-autonomous substrate (Tol2XIG) that was used in this study (not to scale) (Kawakami and Shima, 1999). The Xenopus EF-1a promoter drives expression of GFP. The mini-gene is cloned in the antisense orientation relative to the Tol2 sequence. An intron is included upstream of the reporter to promote correct processing of the transcript. SA, splice acceptor; SD, splice donor. (b) Schematic of the microinjection procedure and subsequent screening. (c) Representative examples of GFP-positive tadpoles after injection of Tol2 plasmid DNA and Tol2 mRNA at the one cell stage. Reproduced with permission of the Publisher, John Wiley & Sons. Image published in: Hamlet MR et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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