XB-IMG-81706
Xenbase Image ID: 81706
|
FIG. 3. Analysis of Smad29,10 and Smad23loxP alleles. a: Embryos from Smad29,10/ intercrosses were observed at E7.5. Smad29,10/9,10 embryos (right, top, and bottom) are much smaller than their wildtype littermate (left). The middle embryo is Smad29,10/, which has embryonic tissue external to the yolk sac. This defect is observed in less than 10% of the heterozygous using the Smad29,10/ or Smad2/ (Liu et al., 2004). b: Xenopus injections were used to compare the function of Smad2 mutant alleles. b: Uninjected embryo control. c: Injection of the dominant- negative Smad2 allele, Smad23SfA. d: Injection of Smad29-11. e: Western blot of injected Xenopus embryos showing the presence of Smad2: flag fusion proteins. The lower panel shows a nonspecific band used for a loading control. f: Smad23loxP/9,10 embryos display a range of phenotypes at E9.5. f: Class I. g: Class II. h: Class III. i: Normal Smad23loxP/ littermate. Scale bar in i 303 m for a; 1.6 mm for f,g; 565 m for h; and 943 m for i. Reproduced with permission of the Publisher, John Wiley & Sons. Image published in: Liu Y et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |