Xenbase Image ID: 81786

Fig. 7. Expression of endocrine and exocrine hormones in stage 48 and stage 50 Xenopus tadpoles. A all show sagittal sections of stage 48 tadpoles stained with different primary antibodies. A) Insulin (green) and glucagon (red) expression in a stage 48 pancreas. B) Same section as in A with insulin (green) and nuclear DAPI staining (blue). C) Insulin (green) and glucagon (red) expression in a stage 48 pancreas. This sagittal section is more medial than the section in A. D) Same section as in C with glucagon (red) and nuclear DAPI staining (blue). E) Insulin (green) and somatostatin (red) staining in a stage 48 pancreas. Arrows point to somatostatin-positive cells within the stomach and intestinal epithelium. F) Same section as in F with somatostatin (red) and nuclear DAPI staining (blue). G) Insulin (green) and carboxypeptidase A (red) expression in a stage 48 pancreas. H) Section of a stage 48 tadpole in which primary antibodies were not added. I) Sagittal section from a stage 50 tadpole showing expression of insulin (green) and glucagon (red) in the pancreas. Arrow points to an islet-like structure; glucagon-positive cells are located at the periphery of a cluster of insulin-positive cells. J) Section of a stage 48 dissected gut stained with hematoxylin (nuclear) and eosin (cytoplasmic). K) Stained transverse section of a stage 48 tadpole. Abbreviations: Ab, antibody; E, eosin; H, hematoxylin; carbA, carboxypeptidaseA; glu, glucagon; ins, insulin; som, somatostatin; d, duodenum; int, intestine; lv, liver; lu, lung; nc, notochord; oe, oesophagus; p, pancreas; pn, pronephros; s, somite; st, stomach. Image published in: Kelly OG and Melton DA (2000) Copyright © 2000. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue sst.1.S smst, som, somatostatin X. laevis Throughout NF stage 48 pancreas stomach small intestine ins.S ins-a, ins-b, ins1, insulin, insulin I, preproinsulin, xins X. laevis Throughout NF stage 48 pancreas dorsal pancreatic bud gcg.L glucagon, proglucagon I X. laevis Throughout NF stage 48 to NF stage 50 pancreas stomach small intestine cpa1.L carbA, carboxypeptidase A, carboxypeptidaseA, cpa X. laevis Throughout NF stage 48 pancreas Image source: Published Larger Image Printer Friendly View

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