XB-IMG-81787
Xenbase Image ID: 81787
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Fig. 8. Specification of molecular markers in gastrula explants. A) Early
gastrula, stage 10.25, were cut into the following halves: right or left halves,
dorsal or ventral halves, and animal or vegetal halves. B) Late gastrula,
stage 12.5, were cut into the following halves: right or left halves, dorsal or
ventral halves, and anterior or posterior halves. In A and B explants were
cultured until stage 41 and analyzed by RT-PCR for various molecular
markers. Three explants were included in each reaction in triplicate; one
representative sample is shown. A reaction without reverse transcriptase
(2RT) is included as a control for DNase treatment. EF1a is included as a
control for RNA recovery. Abbreviations: A, anterior half; An, animal half;
carbA, carboxypeptidaseA; D, dorsal half; edd, endodermin, EF1a, elongation
factor 1 alpha; L, left half; NCAM, neural cell adhesion molecule; pdx1,
pancreatic-duodenal transcription factor 1; R, right half; P, posterior half; V,
ventral half; Vg, vegetal half. Image published in: Kelly OG and Melton DA (2000) Copyright © 2000. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |