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XB-IMG-81885

Xenbase Image ID: 81885


Fig. 6. Hox2.4 expression requires mesoderm induction. The top part of the panel shows a diagram depicting the conjugate-forming procedure. The bottom section shows the results of RNAse protection assays performed on RNA from the fragments. A, animal fragment alone; V, vegetal fragment alone; AN, animal/vegetal conjugate; N, RNA from normal neurulae incubated for the same time as the conjugate. Cytoskeletal actin was used to normalize the amount of RNA loaded; cardiac actin was used to demonstrate the induction of mesoderm in only the whole neurulae and AN conjugates. A, V, AN, and N lanes contain RNA from the equivalent of five, two, two, and two embryos, respectively.

Image published in: Bittner D et al. (1993)

Copyright © 1993. Image reproduced with permission of the Publisher, John Wiley & Sons.

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