Xenbase Image ID: 81901

Fig. 4. Over-expression of PACSIN2 does not prevent integrin or fibronectin expression. Embryos injected in the both blastomeres at the two cells stage were grown to early gastrula. (A) The protein content of either whole embryos (0.5 embryo equivalent) or their blastocoel fluid (20 blastocoels) was analyzed by western blot using a monoclonal antibody to FN (4H2) and a monoclonal antibody to PACSIN2 (3D8). Non-injected embryos (NI) and the embryos over-expressing PACSIN2 (Wt) express and secrete fibronectin. (B) The embryos were dissociated and cell surface proteins were biotinylated. Immunoprecipitations were performed using monoclonal antibodies against integrin β1 (8C8), α5β1 (P8D4) and αv (P3C12) and the precipitates were analyzed by western blot using streptavidin-peroxidase. The graph represents the quantification of 3 (integrins α5β1 and αv) and 4 (β1-integrin) independent experiments using the Histograms of the Photoshop software. Signal intensities have been normalized to the non-injected control embryos (100%). The error bars correspond to the standard deviation from the mean. None of the differences between injected and non-injected embryos were statistically significant (P > 0.05). Image published in: Cousin H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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