Xenbase Image ID: 81911

Figure 4. Cross-species Dnmt1 proteins can substitute for the function of maternal xDnmt1 depleted by antisense RNA injection. (A) Microcephalic and axis-truncated phenotype of xDnmt1-depleted embryos can be rescued in a dose-dependent manner by coinjection of recombinant human (hDnmt1) or mouse (mDnmt1) Dnmt1 proteins with 520 pg of antisense xDnmt1 RNA. The percentage of resulting abnormal embryos scored at stage 35 was plotted for the experiments indicated underneath the bars. (n) Number of injected two-cell blastulae. Yellow indicates injection of antisense only; blue indicates injection of antisense plus recombinant hDnmt1p protein; light blue indicates injection of antisense plus recombinant mDnmt1p protein. (B) Both proteins (4 pg) cannot rescue the microcephalic phenotype. (C) Coinjection with 12 pg of human or mouse Dnmt1protein in the majority of cases can restore the normal appearance. (D) The rest of the embryos have normal or even enlarged heads, but still a very short axis. (E) hDnmt1 protein (40 pg) coinjected with the antisense RNA results in no-axis phenotype similar to that caused by injection of 12 pg of hDnmt1 protein alone into the animal pole at two-cell stage. (F) Vegetal pole cells are sensitive to the same amount (12 pg) of hDnmt1 protein injected alone. Vegetal injection into two-cell blastulae leads to multiple defects as shown at stage 35. Image published in: Stancheva I and Meehan RR (2000) Copyright © 2000. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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