XB-IMG-81955
Xenbase Image ID: 81955
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Fig. 8. Orientation of late gene expression recapitulates the polarity of the NM junction. (A) Diagram to illustrate the neural plate rotations used to generate an extra tail with reversed orientation of N and M. (A) A region of the neural plate 600 600 mm is peeled from an embryo at stage 14. The region
removed is within 5000 mm of the blastopore and therefore contains not only neural plate (N) but a small amount of mesodermal tissue continuous with the
neural plate (M). (B) This square of tissue is rotated 180before replacing it onto the same embryo. (C,D) The displaced NM region, which has already
interacted with C, is now in reverse orientation with M anterior to N, and a second NM junction has been made over C which generates a new tail at the
posterior of the embryo with the correct orientation. Arrows show the direction of extension of the two tail buds. (E) Examples of neural plate rotated
embryos stained for lunatic fringe (E), Xwnt3a (F) or Xcad3 (G) transcripts at stage 30. Reverse dorsal ventral polarity staining in the ectopic tail bud is
marked with a black arrow. Image published in: Beck CW and Slack JM (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |