XB-IMG-82098
Xenbase Image ID: 82098
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Fig. 7. Lineage of injected cells and localization of overexpressed proteins on the cell surface. (A) Transverse section of an embryo injected with 1 ng GFP
RNA plus 0.25 ng Xfz3 RNA near the animal pole region. Notice the contribution of injected cells to presumptive neuroectoderm (N) and epidermis. The
neural tube did not form due to expression of Xfz3. (B) Section made from (A) showing the nuclear and cytoplasmic localization of GFP. (C) Expression of
GFP-tagged Xfz3 (with six transmembrane segments) on the cell surface. Two-cell stage embryos were injected with 1 ng RNA encoding GFP-tagged Xfz3
and sections were made at neurula stages. Notice the presence of GFP-tagged Xfz3 on the cell surface (arrowheads). Scale bars: (A) 350 mm, (B) 60 mm, (C)
35 mm. Image published in: Shi DL et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |