XB-IMG-82106
Xenbase Image ID: 82106
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Fig. 1. Induction of synchronous cell death in embryos. Early cell divisions, in synchronously fertilized eggs, were monitored by time lapse video recordings.
(A) Panels 1 compare a control (right), and irradiated (left) embryo. Embryos were g-irradiated (40 Gy), 1 h post fertilization. Panel 1, St. 2; panel 2, St. 3;
panel 3, St. 5; panel 4, St. 7; panel 5, St. 8; panel 6, St. 9. (B) Panel 1, control embryo, St. 10.5; panel 2, dying irradiated embryo, St. 10.5; panel 3, dying
cycloheximide-treated embryo. This embryo was treated with cycloheximide at the 2-cell stage, at a concentration of 0.1 mg/ml which inhibited protein
synthesis by 97%, cell division arrested at the 4-cell stage, and death occurred at a time equivalent to St. 10.5; panel 4, dying a-amanitin-treated embryo, St.
10.5. Death is defined as the rapid disintegration of the embryo characterized by a white mottled appearance. All treated embryos showed this phenotype. Image published in: Hensey C and Gautier J (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |