XB-IMG-82110
Xenbase Image ID: 82110
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Fig. 5. Embryonic death at St. 10.5 is only induced by treatment prior to the MBT. (A) Embryos were treated with cycloheximide at various stages during
development leading to an arrest in cell division at the following Stages: panels 1 and 2, St. 3; panels 3 and 4, St. 6.5; panels 5 and 6, St. 8; panels 7 and 8, St.
9. Embryos were fixed and TUNEL stained, at times in development equivalent to St. 9 and St. 10.5 in control embryos. Panels 1, 3, 5 and 7 show St. 9
equivalent embryos, panels 2, 4, 6 and 8 show St. 10.5 equivalent embryos. Arrows in panel 2 indicate 3 stained nuclei in a 4-cell embryo. (B) Percent
survival curve of embryos irradiated at different stages during development. Embryos received 40 Gy, St. 1 irradiated (X); St. 7 irradiated (B); St. 8 irradiated
(O); St. 9 irradiated (W). Percent survival refers to the % of surviving embryos in a population, i.e. embryos that do not turn white, or embryos that do not
have greater than 50% of the cell on their surface appearing white. Percentages were scored on batches of 50 embryos, and the data are representative of three
separate experiments. Image published in: Hensey C and Gautier J (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |