XB-IMG-82122
Xenbase Image ID: 82122
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Fig. 3. ADMP and Nodal compete on ACVR2a. (A) Schematic drawing
depicting Nodal and ADMP sharing ACVR2a. (B) Embryos were radially
injected in the marginal zone at the four-cell stage with mature miR-15
(15 ng/embryo) or ACVR2a mRNA (10, 100, or 300 pg/embryo). (B) Neurula-stage embryos were stained for xRx-1. (G) Close up picture of anterior/head region of sibling embryos of B, grown until tadpole stage (stage 37). Pictures are the representatives of >20 embryos from two independent experiments. Frequency of the shown phenotype was >80%. (L) Dorsal overexpression of ACVR2a increases both ADMP- and Nodal-dependent transcriptional activity. Embryos were injected in the dorsal marginal zone with either ID1-luciferase reporter or Mix.2 luciferase reporter with or without AVCR2a (300 pg) mRNA. Targeting to dorsal mesoderm was confirmed at gastrula by coinjected GFP mRNA. Data are given as mean and SD. (M) Overexpression of Xnr1 outcompetes ADMP if ACVR2a levels are limiting. Embryos were injected in the animal pole with the ADMP-responsive ID1-luciferase reporter alone (control) or with combinations of ADMP (100
pg/embryo), Xnr1 (100 pg/embryo), and ACVR2a (300 pg) mRNAs. Data are given as mean and SD. (N) ADMP does not outcompete Xnr1. Embryos were injected in the animal pole with the Nodal-responsive Mix.2-luciferase reporter alone (control) or with combinations of the indicated mRNAs (same doses as in L). Image published in: Inui M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |