XB-IMG-82123
Xenbase Image ID: 82123
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Fig. 4. Cerberus facilitates ADMP signaling by relieving Nodal competition
for ACVR2a. (A) In situ hybridization of Sizzled (Szl) indicates the activities
of ADMP and BMP2. Embryos were radially injected in the marginal zone
at the four-cell stage with the indicated combinations of ADMP mRNA (30
pg/embryo) BMP2 mRNA (20 pg/embryo), CerS mRNA (30 pg/embryo), and
Cerberus + Lefty MOs (60 ng/embryo). Representative embryos are shown
dorsal side up and Sizzled expression domains are highlighted with dotted
lines. To quantify expansions or reduction, the angle of the Sizzled ventral
crescent was measured in >20 embryos (from two independent experiments)
as quantifications are shown in the Right columns with SD. Note that fulllength
Cerberus does not affect ADMP activity, whereas it inhibits BMP responsiveness
(Fig. S6). Image published in: Inui M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |