XB-IMG-82126
Xenbase Image ID: 82126
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Fig. S2. (A) Embryos were injected with dominant-negative Smad5 mRNA (DN-Smad5; 100 pg/embryo) into the marginal zone of dorsal (D) or ventral G) blastomeres at the four-cell stage and expression pattern of neural genes (xAg1, xRx-1, Krox20, and HoxB9) were visualized by in situ hybridization at tail bud stage. Note that the anterior structure is expanded in dorsally injected embryos (red brackets). (A, D, and G) Lateral view. (B, E, and H) Dorsal view. (C, F, and I) Ventral view. Pictures are the representatives of >20 embryos from two independent experiments. Frequency of the shown phenotype was >85%. Image published in: Inui M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |