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Fig. S4. Embryos were radially injected with indicated MOs and mRNA and head/anterior structure was examined at late neurula stage. (A) Close-up picture of head/anterior phenotype. (D) Quantification of frequency of small head phenotype. Number of embryos examined are 26 for control morpholino (CoMO), 32 for CoMO + ACVR2a, 31 for ADMP MO + ACVR2a. (E) Close-up pictures of head/anterior phenotypes at late neurula stage of embryos injected with the indicated mRNAs. Number of embryos (n) was ≥25, derived from two independent experiments; representative pictures are shown. ACVR2a (100 pg) mRNA; hDKK1 (10 pg) mRNA. Image published in: Inui M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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