Xenbase Image ID: 82131

Fig. S7. (A) Embryos injected as Fig. 4 A, but in suboptimal doses (ADMP mRNA 5 pg/embryo, BMP2 mRNA 5 pg/embryo, CerS 30 pg/embryo) were grown to tadpole stage (higher doses of Cer-S mRNA induce cyclopia) (1). Frequency of embryos lacking eyes were (J) 0%n = 20, (K) 0%n = 22, (L) 0%n = 22, (M) 0% n = 21, (N) 60% n = 20, (O) 0% n = 21. (G) Expression of head-organizer genes Cerberus (Cer) and DKK-1 in hemisectioned embryos injected with the indicated MOs (50 ng/embryo). Note that upon depletion of Nodal antagonists the anterior border of the head organizer expands toward the anteriormost endoderm, phenocopying loss of ADMP. Image published in: Inui M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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