XB-IMG-82136
Xenbase Image ID: 82136
Fig. 4. Morphological defects observed in embryos injected with Livertine. (A) Side view of a stage -36 tadpole injected with plasmids driving the
expression of Livertine into dorsal blastomeres at the 4-cell stage. The arrow points to the head region which is deformed and small, a, anterior; p,
posterior. (B) Cross section through the hindbrain region of an embryo similar to that shown in (A) displaying a ventrally displaced notochord (n). The
arrow points to the presence of axons at the ventral midline of the neural tube. The ventral displacement of the notochord lead to the absence of floor
plate differentiation anteriorly. This was confirmed by labeling with anti-HNF-3fl antibodies (not shown), ov, otic vesicle. (C) Embryos injected with
Livertine (bottom) showing the presence of an open blastopore (arrow). The embryo on top is a normal uninjected control. The middle row shows
dorsal views whereas the bottom row shows side views of affected embryos injected with Livertine. Head and tail structures were sometimes detected.
Scale bar: (A) 260/zm; (B) 60/~m; (C) 440/~m. Image published in: Ruiz i Altaba A and Théry C (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |