Xenbase Image ID: 82170

Fig. 2. Spatiotemporal expression of Xmab21l3. (A) Xmab21l3 expression is preceded by the onset of Xema expression. RT-PCR analysis of embryos collected at the indicated stages. The Spemann organizer marker chordin is first detected at stage 9 and increases dramatically at the initiation of gastrulation at stage 10. (B) RT-PCR analysis of embryos collected at the indicated stages. (C) RT-PCR of Xmab21l3 in explants of gastrula stage embryos. Xmab21l3 is expressed predominantly in the animal cap (AC) explants and to a lesser extent in ventral marginal zone (VMZ) explants of stage 10 embryos. DMZ, dorsal marginal zone. (D, D′) Xmab21l3 is expressed in the animal pole ectoderm of gastrula stage embryos. Animal and lateral views of whole mount in situ hybridization analysis of gastrula stage embryos. (E′) Xmab21l3 is excluded from the dorsal neural tube (arrowheads). (E) Dorsal view of stage 20 embryos with anterior to the left. (E′) Posterior view of stage 20 embryos with anterior to the top. (E′) Transverse cross-section of the dorsal neural tube. Dorsal is to the top. Image published in: Sridharan J et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue mab21l3.S mab21-l3, Target of Xema1, Tx1, Xmab21l3 X. laevis Throughout NF stage 10.5 ectoderm animal cap mab21l3.S mab21-l3, Target of Xema1, Tx1, Xmab21l3 X. laevis Throughout NF stage 20 ectoderm non-neural ectoderm Image source: Published Larger Image Printer Friendly View

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