XB-IMG-82171
Xenbase Image ID: 82171
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Fig. 3. Xmab21l3 misexpression promotes dorsalization. (A) Overexpression of Xmab21l3 in embryos causes head defects and ectopic tail-like lateral protrusions. Lateral (top) and dorsal (bottom) views of stage 35 embryos injected with 1 ng of Xmab21l3 RNA in the animal pole of both blastomeres at the 2-cell stage. (B) Xmab21l3-induced lateral protrusions are positive for a somite-specific antigen. Whole mount immunohistochemistry of Xmab21l3 RNA-injected tadpoles with the 12/101 antibody (Kintner and Brockes, 1984). Arrows in (A) indicate protrusions; arrowheads in (B) indicate secondary axes. (C) Xmab21l3 overexpression promotes induction of dorsal markers chordin and goosecoid by Activin. RT-PCR analysis of uninjected and Xmab21l3 RNA-injected animal cap explants cultured until stage 10.5 in the absence or presence of a low dose of Activin (5 ng/ml). (D) Xmab21l3 overexpression induces neural markers sox2 and sox3 in competent ectoderm. RT-PCR analysis of animal cap explants from Xmab21l3 RNA-injected embryos cultured until stage 18. Xmab21l3 (l) and Xmab21l3 (h) indicate 0.5 ng/embryo and 1 ng/embryo doses, respectively, of injected Xmab21l3 RNA. Image published in: Sridharan J et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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