Xenbase Image ID: 82173

Fig. 5. Loss of function of Xmab21l3 causes anterior defects and lethality. (A) Xmab21l3MO blocks translation of a 3′Myc-tagged Xmab21l3 protein in a dose-dependent manner. Western blot analysis was used to detect Myc-tagged Xmab21l3 protein. (B) Loss of Xmab21l3 affects eye development and axis formation. Low doses of Xmab21l3 MO (2.5 ng) leads to a loss of eyes and/or mild axis curvature in a subset of embryos (middle). Higher doses of Xmab21l3 MO (6.4 ng) give rise to pronounced head defects and shortened body axes (right). Embryos in left panel were injected with 20 ng of scrambled (control) MO. (C) Coinjection of 1 ng RNA encoding a Xmab21l3MO-insensitive silent mutant (Xmab21l3SMT) decreases the percentage of embryos with severe phenotypes. (D) High levels of Xmab21l3MO (250 ng) cause lethality in 95% of gastrula stage embryos. Survival can be rescued in a dose-dependent manner by injection of Xmab21l3SMT. (C, D) Injection of a 5 base-pair mismatch (5MM) control morpholino does not give rise to developmental defects or lethality. Representative experiments shown in C, D; n ⩾ 15 for each condition. Image published in: Sridharan J et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + mab21l3 MO + NF35/36 (morphology) fig.5.b Anatomical Phenotype abnormal anterior-posterior axis morphology absent eye Xla Wt + mab21l3 MO + NF35/36 (morphology) fig.5.b Anatomical Phenotype absent eye decreased length of anterior-posterior axis decreased size of the head decreased size of the tail Xla Wt + mab21l3 MO + gastrula stage (morphology) fig.5.d Anatomical Phenotype abnormally semi-viable whole organism Larger Image Printer Friendly View

Return to previous page