XB-IMG-82189
Xenbase Image ID: 82189
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FIG. 1. MO based inhibition of GFP fluorescence in transgenic X. laevis and X. tropicalis. (a) CMV- GFP transgenic X. laevis (top, F2, single integra- tion (Marsh-Armstrong et al., 1999); and X. tropicalis (bottom, F1, multiple integrations, gen- erated as previously described (Kroll and Amaya, 1996) (b) Wild-type embryo injected at the two- cell stage into one blastomere with 10 ng FITC labeled control MO (5-CCTCTTACCTCAGTTA- CAATTTATA-3; gene tools). (c) CMV-GFP trans- genic embryos injected into one cell at the two-cell stage with 2 ng gfp-MO (5-AAGTTCT- TCTCCTTTACTCATGGTG-3; gene tools). (d) Dose-dependent inhibition of GFP. Both blas- tomeres at the two-cell stage were injected with gfp-MO to give the indicated total dose (ng). (e) Embryos were injected with 1 ng gfp-MO as in (c) and one individual was followed through- out development. (i) X. laevis CryGFP3 (F2, single integration (Bronchain et al., 1999); and (j) X. tropicalis CryGFP3 (F2, single integration (Offield et al., 2000); transgenic embryos were injected as indicated (ng). +/- presence or absence of transgene. Image published in: Nutt SL et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |