Xenbase Image ID: 82244

FIGURE 5. Overexpression of frog rdhe2 in Xenopus embryos. A, wild-type or mRNA-injected embryos treated with all-trans-retinol and incubated until early tadpole stages, fixed, and divided into severe, moderate, mild, and normal groups according to the severity of manifested defects. The figure shows representative embryos for each phenotypic class. B, proportion of embryos manifesting defects of different severity after injections of rdhe2 mRNA (4 ng/embryo), raldh2 mRNA (1 ng), or a mixture of both. Embryos were treated with 5 or 8 μM all-trans-retinol from stage 8 to stage 14. C, expression of anterior marker genes otx2, krox20, en2, and rx2a in injected (arrow) versus uninjected side of the embryo analyzed by whole mount in situ hybridization. Image published in: Belyaeva OV et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 eye primordium optic vesicle otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 eye primordium optic vesicle forebrain egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 14 neural crest presumptive rhombomere nervous system cranial neural crest hindbrain en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 14 brain midbrain Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + aldh1a2 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + aldh1a2 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + aldh1a2 + sdr16c5 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + aldh1a2 + sdr16c5 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally incomplete structure of head decreased size of the eye Xla Wt + sdr16c5 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + sdr16c5 + retinol + NF32-32 (morphology) fig.5.a, b Anatomical Phenotype abnormally anteriorly mislocalised eye abnormally incomplete structure of head absent eye decreased length of anterior-posterior axis fused eyes Xla Wt + sdr16c5 + retinol + NF32-32 (morphology) fig.5.b Anatomical Phenotype abnormal NAD-retinol dehydrogenase activity Xla Wt + sdr16c5 + NF32-32 (in situ hybridization) fig.5.c Expression Phenotype mislocalised egr2.L expression in presumptive rhombomere mislocalised en2.L expression in midbrain decreased amount otx2.S expression in eye primordium decreased amount rax.L expression in eye primordium Larger Image Printer Friendly View

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