Xenbase Image ID: 82246

FIGURE 7. Decreased retinoid signaling in rdhe2 morphants. A, rdhe2- or control-MO (155 ng) mixed with the lineage tracer were injected into one dorsal blastomere at four-cell stage. The injected side is indicated by an arrow. D, rdhe2- or control-MO (50 ng/embryo) was injected into two dorsal blastomers. D, embryos were treated with 0.01 or 0.1 μM RA where specified. A, whole mount in situ hybridization with antisense RNA probes was performed for the following markers (stages and frequencies of observed phenotypes in parentheses): A, otx2 and krox20 (st. 20, 31/77); B, en2 (st. 189, 42/74); C and D, hoxd4 (st. 189, 30/46); E, sox3 (st. 134, 10/12); F, shh (st.14, 23/28); G, myod (st. 302, 15/42). Arrows in G indicate irregular somites as revealed by myod staining. H, tissues of rdhe2 and control morphants were incubated over the monolayer of F9-RARE-lacZ cells; β-galactosidase activity was revealed with X-gal substrate. The results are representative of three independent experiments. Image published in: Belyaeva OV et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 20 brain forebrain eye primordium egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 20 hindbrain neural crest presumptive rhombomere en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Sometime during NF stage 18 to NF stage 19 brain midbrain hoxd4.L LOC108703007 X. laevis Sometime during NF stage 18 to NF stage 19 hindbrain neural fold ectoderm sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Sometime during NF stage 13 to NF stage 14 neural plate pre-chordal neural plate chordal neural plate preplacodal ectoderm shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 14 notochord myod1.L mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Sometime during NF stage 29 and 30 to NF stage 32 somite trunk tail presomitic mesoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + sdr16c5 MO + NF20 (in situ hybridization) fig.7.a Expression Phenotype decreased amount egr2.L expression in neural crest decreased amount egr2.L expression in presumptive rhombomere mislocalised egr2.L expression in presumptive rhombomere decreased amount otx2.S expression in eye primordium mislocalised otx2.S expression in eye primordium Xla Wt + sdr16c5 MO + NF18-19 (in situ hybridization) fig.7.b Expression Phenotype mislocalised hoxd4.L expression in hindbrain decreased amount hoxd4.L expression in hindbrain decreased amount hoxd4.L expression in neural fold Xla Wt + RA + NF18-19 (in situ hybridization) fig.7.d Expression Phenotype mislocalised hoxd4.L expression in hindbrain increased amount hoxd4.L expression in hindbrain Xla Wt + sdr16c5 MO + NF18-19 (in situ hybridization) fig.7.d Expression Phenotype mislocalised hoxd4.L expression in hindbrain decreased amount hoxd4.L expression in hindbrain decreased amount hoxd4.L expression in neural fold Xla Wt + sdr16c5 MO + NF13-14 (in situ hybridization) fig.7.e Expression Phenotype increased amount sox3.S expression in neural plate Xla Wt + sdr16c5 MO + NF14 (in situ hybridization) fig.7.f Expression Phenotype decreased amount shh.L expression in notochord increased amount shh.L expression in notochord Xla Wt + sdr16c5 MO + NF29/30-32 (in situ hybridization) fig.7.g Anatomical Phenotype abnormal development of somite abnormally irregular spatial pattern of somite Larger Image Printer Friendly View

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