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Fig. 5. Smurf1 and Smurf2 differentially regulate mesodermal markers at gastrula stages. A: Overexpression of Smurf2 in either DMZ or VMZ disrupts expression of the pan-mesodermal marker Brachyury (XBra). Dorsal expression of Smurf2 slightly reduces the dorsal mesoderm marker chordin, whereas ventral expression of Smurf2 inhibits the ventral-lateral mesodermal marker wnt8. B: Unlike Smurf2, Smurf1 does not block, but instead mildly expands, the expression of XBra or chordin, though like Smurf2, it also inhibits wnt8 expression. Co-expression of Smurf1 and 2 leads to reduction of all three mesodermal markers. The numbers indicate the embryos displaying the shown changes in marker expression. Image published in: Das S and Chang C (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 mesoderm marginal zone involuting marginal zone wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 10.5 ventro-lateral marginal zone chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 dorsal marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + smurf2 + NF10.5 (in situ hybridization) fig.5.a Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in dorsal marginal zone decreased amount tbxt.S expression in ventral marginal zone decreased amount wnt8a.L expression in ventro-lateral marginal zone Xla Wt + smurf1 + NF10.5 (in situ hybridization) fig.5.b Expression Phenotype increased amount chrd.1.S expression in dorsal marginal zone increased amount tbxt.S expression in dorsal marginal zone decreased amount wnt8a.L expression in ventro-lateral marginal zone Xla Wt + smurf1 + smurf2 + NF10.5 (in situ hybridization) fig.5.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in dorsal marginal zone decreased amount wnt8a.L expression in ventro-lateral marginal zone Xla Wt + smurf2 + NF10.5 (in situ hybridization) fig.5.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in dorsal marginal zone decreased amount wnt8a.L expression in ventro-lateral marginal zone Larger Image Printer Friendly View

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