Xenbase Image ID: 82286

Fig. 6. Smurf2 antisense morpholino oligonucleotides (Smurf2-MO) acts specifically to block translation and function of Xenopus Smurf2. A: Smurf2 antibody recognizes Xenopus Smurf2, but not Smurf1. B: Smurf2-MO inhibits translation from the RNA encoding Xenopus Smurf2 (XSmurf2), but has no effect on translation of human Smurf2 (hSmurf2). C: Smurf2-MO blocks induction of a partial secondary axis by XSmurf2, but not by hSmurf2. D: Smurf2-MO reduces endogenous Smurf2 protein levels at the gastrula stages. E: Smurf1-MO does not regulate the protein level of Smurf2. F: Ectodermal expression of Smurf2-MO induces head defects, which are partially rescued by low doses of co-expressed hSmurf2. Image published in: Das S and Chang C (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xla Wt + Hsa.smurf2 + NF33/34 (morphology) fig.6.c Anatomical Phenotype obsolete duplicated anterior-posterior axis wholly anteriorized embryo Xla Wt + smurf2 + NF33/34 (morphology) fig.6.c Anatomical Phenotype obsolete duplicated anterior-posterior axis Xla Wt + Hsa.smurf2 + NF33/34 (morphology) fig.6.f Anatomical Phenotype decreased size of the eye Xla Wt + smurf2 MO + NF33/34 (morphology) fig.6.f Anatomical Phenotype abnormal eye morphology decreased length of anterior-posterior axis decreased size of the head Larger Image Printer Friendly View

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