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Fig. 8. Smurfs regulate ectodermal marker expression. A: Knockdown of Smurf2 slightly reduced the neural marker expression and expanded the epidermal marker XK70, but the neural plate border genes Slug and Pax3 could be both up- and down-regulated in the morphants. B: Knockdown of Smurf1 resulted in slightly reduced Sox2 in a wider domain and decreased Pax6 expression; Slug was also predominantly reduced (10/54 and 35/54 embryos with enhanced and decreased Slug expression, respectively). Depletion of both Smurf1 and Smurf2 caused reduction and disorganization of the Sox2 domain, and decreased expression of both Pax6 and Slug (8/50 and 36/50 embryos, respectively, with enhanced and reduced Slug in 25ng combination of both MOs, and no embryos showed higher expression of Slug in double morphants with 50 ng combination of the MOs). Image published in: Das S and Chang C (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 18 neural plate neural fold eye primordium nrp1.L A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 X. laevis Throughout NF stage 18 neural plate neural plate border sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural plate border krt70.S krt, krt-a, krt-b, xk70 X. laevis Throughout NF stage 18 ectoderm epidermis non-neural ectoderm pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 18 neural plate border neuroectoderm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + smurf2 MO + neurula stage (in situ hybridization) fig.8.a Expression Phenotype increased amount krt70.S expression in epidermis decreased amount nrp1.L expression in neural plate increased amount pax3.S expression in neural plate decreased amount pax3.S expression in neural plate decreased amount pax6.L expression in neural plate increased amount snai2.L expression in neural plate decreased amount snai2.L expression in neural plate decreased amount sox2.L expression in neural plate Xla Wt + smurf1 MO + neurula stage (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in neural plate decreased amount snai2.L expression in neural plate increased amount snai2.L expression in neural plate increased amount sox2.L expression in neural plate decreased amount sox2.L expression in neural plate Xla Wt + smurf2 MO + neurula stage (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in neural plate decreased amount snai2.L expression in neural plate increased amount snai2.L expression in neural plate decreased amount sox2.L expression in neural plate Xla Wt + smurf2 MO + neurula stage (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in neural plate decreased amount snai2.L expression in neural plate Xla Wt + smurf2 MO + neurula stage (in situ hybridization) fig.8.b Expression Phenotype decreased amount pax6.L expression in neural plate decreased amount snai2.L expression in neural plate increased amount snai2.L expression in neural plate decreased amount sox2.L expression in neural plate Larger Image Printer Friendly View

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