Xenbase Image ID: 82290

Fig. 10. Smurf1 and Smurf2 differentially regulate mesodermal marker expression. A: Knockdown of Smurf2 in the marginal zone region caused expansion of the pan-mesodermal marker XBra towards the ectodermal region. Dorsal knockdown of Smurf2 reduced expression of chordin,whereas ventral knockdown of Smurf2 led to expansion of wnt8 into the ectodermal domain. B: Unlike Smurf2 knockdown, Smurf1 knockdown reduced the expression of both XBra and chordin, and double knockdown of both Smurfs resulted in more efficient reduction of these markers. The numbers indicate the embryos displaying the shown changes of marker expression. Image published in: Das S and Chang C (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 mesoderm marginal zone ventral marginal zone dorsal marginal zone involuting marginal zone wnt8a.L wnt-8, wnt8, Xwnt-8, Xwnt8 X. laevis Throughout NF stage 10.5 ventral marginal zone ventro-lateral marginal zone chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 dorsal marginal zone involuting marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + smurf2 MO + NF10.5 (in situ hybridization) fig.10.a Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone mislocalised tbxt.S expression in dorsal marginal zone increased amount tbxt.S expression in dorsal marginal zone mislocalised tbxt.S expression in ventral marginal zone increased amount tbxt.S expression in ventral marginal zone mislocalised wnt8a.L expression in marginal zone increased amount wnt8a.L expression in marginal zone Xla Wt + smurf1 MO + NF10.5 (in situ hybridization) fig.10.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in marginal zone increased amount wnt8a.L expression in marginal zone Xla Wt + smurf1 MO + smurf2 MO + NF10.5 (in situ hybridization) fig.10.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in marginal zone increased amount wnt8a.L expression in marginal zone Xla Wt + smurf1 MO + smurf2 MO + NF10.5 (in situ hybridization) fig.10.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone decreased amount tbxt.S expression in marginal zone increased amount wnt8a.L expression in marginal zone Xla Wt + smurf2 MO + NF10.5 (in situ hybridization) fig.10.b Expression Phenotype decreased amount chrd.1.S expression in dorsal marginal zone increased amount tbxt.S expression in marginal zone mislocalised tbxt.S expression in marginal zone increased amount wnt8a.L expression in marginal zone Larger Image Printer Friendly View

Return to previous page