Xenbase Image ID: 82315

Figure1. Mitotic Spindle Position in a Developing Epithelium(A) Stills taken from a single focal plane movie of mitotic spindles in the outer epithelial layer of a Xenopus laevis embryo. Embryos were injected with GFP--tubulin (green) to label spindles and Cherry-histone2B (Cherry-H2B) to highlight chromosomes. Spindles are aligned parallel to the plane of the epithelium but are also held in a specific position along the apicobasal axis of the cell; spindles in neighboring cells assemble in the same focal plane and remain here throughout mitosis (arrows).(B) Zoom-in of movie in (A) shows that spindles undergo rapid rotational movement in the x/y plane, while remaining held in parallel orientation and apicobasal position.(C) 3D reconstruction of Cherry-H2B fluorescence from a z stack confocal movie (Movie S2), which can be used to track the apicobasal position of nuclei as mitosis proceeds (side view; apical at top, basal at bottom). Virtually no movements in the apicobasal axis are seen.(D) A side-view image of a mitotic spindle in the outer epithelium of a fixed embryo (apical at top, basal at bottom) demonstrates the apicobasal position of spindles in these cells.(E) Mean values for cell length, width, and distance of spindle from apical surface are shown (SEM, n= 91 spindles in 18 embryos).Scale bars represent 20m in (A) and (B) and 10m in (D). See also Movies S1 and S2. Image published in: Woolner S and Papalopulu N (2012) © 2012 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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