XB-IMG-82324
Xenbase Image ID: 82324
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Figure S3, related to Figure 5: (A) Quantification of interphase nuclei position in Control MO and Myo10 MO injected embryos. Nuclei position was measured relative to cell length. No significant difference in nuclei position was seen (an unpaired Student t-test was performed; n=3 independent experiments from a total of 21 embryos for Ctrl MO and Myo10 MO). (B) To determine Myo10 localisation, embryos were injected with GFP or GFP-HIQT (full length Myo10 tagged with GFP) and stained using an anti-GFP antibody (green). GFP-HIQT localises to the spindle and shows strong accumulation at the cell cortex. (C) Quantification of spindle position following over-expression of Myo10 constructs. In a control background, expression of GFP-HIQT causes spindles to reposition slightly basally, as does expression of GFP-IQT. Whereas, expression of GFP-HIQCC causes spindles to reposition slightly apically when compared to controls. To test for significance, unpaired Student t-tests were performed (n=3 independent experiments, from a total of 20, 19, 18, and 16 embryos each for Ctrl MO + GFP, + GFP-HIQT + GFP-HIQCC and + GFP-IQT, respectively; *p<0.05, **p<0.01). (D) Quantification of metaphase spindle length in Ctrl and Myo10 MO injected embryos, measured relative to cell width. Spindles in Myo10 morphants are significantly longer than controls (an unpaired Student t-test was performed; n=3 independent experiments from a total of 21 embryos for Ctrl MO and Myo10 MO, ***p<0.001). Image published in: Woolner S and Papalopulu N (2012) © 2012 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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